E. Coli anticorps

N° du produit ABIN110776

Cet anticorps Souris Monoclonal détecte spécifiquement E. Coli dans IF, EIA et WB. Il présente une réactivité envers E. coli.

Aperçu rapide pour E. Coli anticorps (ABIN110776)

Antigène: E. Coli

Reactivité: E. coli

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp E. Coli est non-conjugé

Application: Immunofluorescence (IF), Enzyme Immunoassay (EIA), Western Blotting (WB)

Clone: 2D7-1

Détail du produit anti-E. Coli anticorps

Purification: Affinity Chromatography on Protein A

Immunogène: Escherichia coli J5 cells.

Isotype: IgG2b

Information d'application

Indications d'application: Immunofluorescence. ELISA: (a 1 mg/mL solution has been used at dilutions of up to 1/1500).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur E. Coli

Antigène: E. Coli

Classe de substances: Bacteria

Sujet: Some form of LPS is a major component of the cell membrane of Gram negative bacteria, contributing greatly to the structural integrity of the bacteria, and protecting the membrane from certain kinds of chemical attack. LPS is an endotoxin, and induces a strong response from normal animal immune systems. LPS function has been under experimental research for several years due to its role in activating many transcriptional factors, which become active after stimulation with LPS. LPS also produces many types of mediators involved in septic shock. The J5 mutant of E. coli lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The 55 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella).Synonyms: EHEC, EPEC, ETEC, Escherichia coli